Ju, Tian;
Li, Jixuan;
Weston, Andrew;
Satta, Giovanni;
Bolognini, Sara;
Di Luca, Mariagrazia;
Gaisford, Simon;
(2025)
Anti-Pseudomonas Aeruginosa Bacteriophage Loaded Electrospun Fibers for Antibacterial Wound Dressings.
Macromolecular Rapid Communications
, Article 2400744. 10.1002/marc.202400744.
(In press).
Preview |
Text
Tian phages MS.pdf - Published Version Download (3MB) | Preview |
Abstract
Antimicrobial resistance poses a growing threat to public health globally. Multidrug resistant Pseudomonas (P.) aeruginosa is detected in many infected wounds and is very challenging to treat with antibiotics. An alternative to antibiotics is to use bacteriophages, highly specific viruses able to kill even resistant bacteria. This work incorporates anti-P. aeruginosa Neko phages into monoaxial and coaxial electrospun fibers to explore their potential for treating infected wounds. Phages are blended with polyvinyl alcohol (PVA) solution and either processed directly into fibers or used as the core in coaxial electrospinning with polyvinylpyrrolidone (PVP) and PVP/ethyl cellulose (EC) shell solutions. Coaxial fibers stored at −20 °C show promising stability results, with negligible phage titer loss after 6 months of storage. Phage release can be controlled by varying the shell composition. Coaxial fibers with PVP as the shell (PVA/Su PVP + phage) demonstrate immediate release while fibers with PVP/EC as the shell (PVA/Su PVP/EC + phage) display extended-release. The antibacterial efficacy of phage lysate and phage-loaded fibers is studied by isothermal calorimetry and found to be unaffected by electrospinning. Thus, it appears that phage-loaded electrospun fibers merit further investigation as potential wound dressing materials.
| Type: | Article |
|---|---|
| Title: | Anti-Pseudomonas Aeruginosa Bacteriophage Loaded Electrospun Fibers for Antibacterial Wound Dressings |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/marc.202400744 |
| Publisher version: | https://doi.org/10.1002/marc.202400744 |
| Language: | English |
| Additional information: | Copyright © 2025 The Author(s). Macromolecular Rapid Communications published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, https://creativecommons.org/licenses/by/4.0/, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Bacteriophage, coaxial electrospinning, ethyl cellulose (EC), isothermal calorimetry, polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), pseudomonas aeruginosa |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10204097 |
Loading...
Loading...Loading...Loading...Archive Staff Only
 |
View Item |
