Picco, Francesca;
Zeboudj, Lynda;
Oggero, Silvia;
Prato, Vincenzo;
Burgoyne, Thomas;
Gamper, Nikita;
Malcangio, Marzia;
(2025)
Macrophage to neuron communication via extracellular vesicles in neuropathic pain conditions.
Heliyon
, 11
(1)
, Article e41268. 10.1016/j.heliyon.2024.e41268.
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Abstract
Neuropathic pain following peripheral nerve injury results from maladaptive changes in neurons and immune cells contribution to mechanisms underlying chronic pain. Specifically, in dorsal root ganglia (DRG), sensory neuron cell bodies release extracellular vesicles (EVs) which promote pro-inflammatory macrophage accumulation that facilitates nociceptive signalling. Here, we show that macrophages shuttle EVs to neurons. Indeed, bone marrow-derived macrophages (BMDMs) release EVs containing microRNA-155 (miR-155) which are taken up by cultured sensory neurons. EV-mediated transfer of miR-155 suppresses phosphatase Ship1 expression and increases cytokine interleukin-6 (IL-6) contents. Intrathecal-injected BMDM-derived EVs accumulate in lumbar DRG and EVs containing miR-155 antagomir result in Ship1 upregulation, Il6 downregulation in neurons in concomitance to attenuation of neuropathic mechanical hypersensitivity. These data suggest that, under neuropathic conditions, pro-inflammatory macrophages shuttle EV-containing miR-155 to neurons and contribute to the expression of pronociceptive IL-6 in DRG.
| Type: | Article |
|---|---|
| Title: | Macrophage to neuron communication via extracellular vesicles in neuropathic pain conditions |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.heliyon.2024.e41268 |
| Publisher version: | https://doi.org/10.1016/j.heliyon.2024.e41268 |
| Language: | English |
| Additional information: | © 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Neuropathic pain, DRG neurons, Macrophages, Extracellular vesicles, microRNAs, Cytokines |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10202807 |
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