Mathers, Amy;
Li, Thomas;
He, Qijan;
Narendra, Sharvari;
Stoesser, Nicole;
Eyre, David;
Walker, Ann;
... Reidys, Christian; + view all
(2024)
Developing a framework for tracking antimicrobial resistance gene movement in a persistent environmental reservoir.
npj Antimicrobials and Resistance
, 2
, Article 50. 10.1038/s44259-024-00069-w.
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Abstract
Mobile genetic elements are key to the global emergence of antibiotic resistance. We successfully reconstructed the complete bacterial genome and plasmid assemblies of isolates sharing the same blaKPC carbapenemase gene to understand evolution over time in six confined hospital drains over five years. From 82 isolates we identified 14 unique strains from 10 species with 113 blaKPC-carrying plasmids across 16 distinct replicon types. To assess dynamic gene movement, we introduced the ‘Composite-Sample Complex’, a novel mathematical approach to using probability to capture the directional movement of antimicrobial resistance genes. The Composite Sample Complex accounts for the co-occurrence of both plasmids and chromosomes within an isolate, and highlights likely gene donors and recipients. From the validated model, we demonstrate frequent transposition events of blaKPC from plasmids to other plasmids, as well as integration into the bacterial chromosome within specific drains. We present a novel approach to estimate the directional movement of antimicrobial resistance via gene mobilization.
| Type: | Article |
|---|---|
| Title: | Developing a framework for tracking antimicrobial resistance gene movement in a persistent environmental reservoir |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s44259-024-00069-w |
| Publisher version: | https://doi.org/10.1038/s44259-024-00069-w |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/. |
| Keywords: | Antimicrobial resistance, Microbial ecology, Molecular evolution |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Inst of Clinical Trials and Methodology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Inst of Clinical Trials and Methodology > MRC Clinical Trials Unit at UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10200918 |
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