Memar, Nadin;
Sherrard, Ryan;
Sethi, Aditya;
Fernandez, Carla Lloret;
Schmidt, Henning;
Lambie, Eric J;
Poole, Richard J;
... Conradt, Barbara; + view all
(2024)
The replicative helicase CMG is required for the divergence of cell fates during asymmetric cell division in vivo.
Nature Communications
, 15
(1)
, Article 9399. 10.1038/s41467-024-53715-2.
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Abstract
We report that the eukaryotic replicative helicase CMG (Cdc45-MCM-GINS) is required for differential gene expression in cells produced by asymmetric cell divisions in C. elegans. We found that the C. elegans CMG component, PSF-2 GINS2, is necessary for transcriptional upregulation of the pro-apoptotic gene egl-1 BH3-only that occurs in cells programmed to die after they are produced through asymmetric cell divisions. We propose that CMG's histone chaperone activity causes epigenetic changes at the egl-1 locus during replication in mother cells, and that these changes are required for egl-1 upregulation in cells programmed to die. We find that PSF-2 is also required for the divergence of other cell fates during C. elegans development, suggesting that this function is not unique to egl-1 expression. Our work uncovers an unexpected role of CMG in cell fate decisions and an intrinsic mechanism for gene expression plasticity in the context of asymmetric cell division.
| Type: | Article |
|---|---|
| Title: | The replicative helicase CMG is required for the divergence of cell fates during asymmetric cell division in vivo |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41467-024-53715-2 |
| Publisher version: | http://dx.doi.org/10.1038/s41467-024-53715-2 |
| Language: | English |
| Additional information: | © 2024 Springer Nature Limited. This article is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Asymmetric Cell Division, DNA Helicases, DNA Replication |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10199570 |
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