Nazmutdinova, Katia;
Man, Cheuk Yan;
Carter, Martyn;
Beales, Philip L;
Winyard, Paul JD;
Walsh, Stephen B;
Price, Karen L;
(2024)
Cell Catcher: A New Method to Extract and Preserve Live
Renal Cells from Urine.
Kidney360
, 5
(9)
pp. 1359-1363.
10.34067/KID.0000000000000503.
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Abstract
A proportion of urinary tract cells are shed into urine by using normal physiological processes. These include epithelial cells from kidney tubules, podocytes, as well as immune and bladder cells.1 Urine-derived cells offer advantages over biopsies because they are easily obtained repeatedly without pain or discomfort. Furthermore, they have various research uses, including modeling genetic kidney disorders,2 generating stem cells,3 and drug screening.4,5 Despite these advantages, the full potential of urine-derived cells is not being realized. One key issue is inconsistencies between methods to initiate cell cultures from urine. Healthy adult urine contains between 2.5 and 7.5 cells/100 ml, which can proliferate in culture, yielding millions of cells within 2–4 weeks.6,7 However, the success rate of initiating and expanding cells from urine is variable, ranging from 10% to 73%.3,8–10 In addition, the cell population obtained is heterogeneous, containing both differentiated and undifferentiated cells,11 complicating the interpretation of studies. These inconsistencies in yields and cell identity are likely due to methodological differences, including culture conditions. Currently, urinary cells are isolated within 4 hours of sample collection, using two-step centrifugation, requiring a laboratory in close proximity to the collection site. This makes the process logistically challenging and extends the time cells are exposed to urine affecting cell viability.1 We hypothesized that immediate processing through filtration will improve the yield of cultured cells obtained from urine compared with centrifugation. This is because filtration minimizes urine exposure time, decreases processing times, and reduces mechanical stress on cells. To test this, we developed a filtration-based Cell Catcher device for processing urine at clinical sites and directly compared its efficiency with centrifugation.
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