Li, Yixuan; (2024) Molecularly imprinted polymer-based electrochemical biosensors for biomarkers detection. Doctoral thesis (Ph.D), UCL (University College London).

Preview

Text Final Copy of Thesis.pdf - Other Download (12MB) | Preview

Abstract

Progressive diseases refer to illness conditions that deteriorate over time, such as chronic kidney disease (CKD), Alzheimer’s disease, etc. Often, these diseases initially present without symptoms, but eventually leading to significant impairment, organ failure, or death. Early intervention is the key to curing, slowing, or stopping the progression of the diseases, significantly improving the life quality of those impacted. Therefore, it is crucial to develop a highly sensitive technique for the quantification of biomarkers in body fluids, realizing the early detection and ongoing monitoring of these progressive diseases. Here, this research first proposed a novel surface imprinting strategy for CKD diagnosis, with molecularly imprinted polymer (MIP)-polydopamine (PDA) coating on graphene nanoplatelets (GNP) for the quantification of creatinine biomarker in human serum, urine, and peritoneal dialysis (PD) fluids, showing a low limit-of-detection (LoD) along with excellent sensitivity, selectivity, and accuracy over a wide dynamic range. Next, based on this promising surface imprinting strategy, the composite was further optimized to use reduced graphene oxide (rGO)/PDA-MIP for Alzheimer’s disease diagnosis, achieving ultratrace detection of glial fibrillary acidic protein (GFAP) at an attomolar level in human plasma and cerebrospinal fluid (CSF). Also, considering the growing need for the point-of-care (POC) testing, the fabricated composite was combined with a cost-effective electronic readout platform with differential pulse voltammetry (DPV) function, allowing Alzheimer’s disease screening and progression monitoring by primary healthcare workers. At last, considering that the detection of a single biomarker is not necessarily informative enough to establish a clinical diagnosis or track disease progression, the above strategy was utilized to fabricate corresponding MIPs and combine with a multi-channel readout platform with function of DPV to realize the simultaneous detection of CKD biomarkers including creatinine, urea, and human serum albumin (HSA). This POC sensing system shows remarkably high sensitivity, selectivity, reproducibility, stability, and record LoD at femtomolar level with wide linear range when tested in human serum and urine samples, which makes it a promising approach for screening for diseases and monitoring progression, especially in resource-limited regions.

Download activity - last month

Export as XMLJSONCSV

Download activity - last 12 months

Export as JSONCSVXML

Downloads by country - last 12 months

Export as XMLJSONCSV

Archive Staff Only

View Item