UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Prospective Role of PAK6 and 14-3-3γ as Biomarkers for Parkinson’s Disease

Giusto, Elena; Maistrello, Lorenza; Iannotta, Lucia; Giusti, Veronica; Iovino, Ludovica; Bandopadhyay, Rina; Antonini, Angelo; ... Civiero, Laura; + view all (2024) Prospective Role of PAK6 and 14-3-3γ as Biomarkers for Parkinson’s Disease. Journal of Parkinson's Disease , 14 (3) pp. 495-506. 10.3233/JPD-230402. Green open access

[thumbnail of Bandopadhyay_jpd_2024_14-3_jpd-14-3-jpd230402_jpd-14-jpd230402.pdf]
Preview
Text
Bandopadhyay_jpd_2024_14-3_jpd-14-3-jpd230402_jpd-14-jpd230402.pdf

Download (916kB) | Preview

Abstract

Background: Parkinson’s disease is a progressive neurodegenerative disorder mainly distinguished by sporadic etiology, although a genetic component is also well established. Variants in the LRRK2 gene are associated with both familiar and sporadic disease. We have previously shown that PAK6 and 14-3-3γ protein interact with and regulate the activity of LRRK2. // Objective: The aim of this study is to quantify PAK6 and 14-3-3γ in plasma as reliable biomarkers for the diagnosis of both sporadic and LRRK2-linked Parkinson’s disease. // Methods: After an initial quantification of PAK6 and 14-3-3γ expression by means of Western blot in post-mortem human brains, we verified the presence of the two proteins in plasma by using quantitative ELISA tests. We analyzed samples obtained from 39 healthy subjects, 40 patients with sporadic Parkinson’s disease, 50 LRRK2-G2019S non-manifesting carriers and 31 patients with LRRK2-G2019S Parkinson’s disease. // Results: The amount of PAK6 and 14-3-3γ is significantly different in patients with Parkinson’s disease compared to healthy subjects. Moreover, the amount of PAK6 also varies with the presence of the G2019S mutation in the LRRK2 gene. Although the generalized linear models show a low association between the presence of Parkinson’s disease and PAK6, the kinase could be added in a broader panel of biomarkers for the diagnosis of Parkinson’s disease. // Conclusions: Changes of PAK6 and 14-3-3γ amount in plasma represent a shared readout for patients affected by sporadic and LRRK2-linked Parkinson’s disease. Overall, they can contribute to the establishment of an extended panel of biomarkers for the diagnosis of Parkinson’s disease.

Type: Article
Title: Prospective Role of PAK6 and 14-3-3γ as Biomarkers for Parkinson’s Disease
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.3233/JPD-230402
Publisher version: http://dx.doi.org/10.3233/jpd-230402
Language: English
Additional information: Copyright © 2024 – The authors. Published by IOS Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC 4.0).
Keywords: PAK6, 14-3-3, Parkinson’s disease, biomarkers
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences
URI: https://discovery.ucl.ac.uk/id/eprint/10191257
Downloads since deposit
9Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item