Pelliciari, Simone;
Bodet-Lefevre, Salome;
Fenyk, Stepan;
Stevens, Daniel;
Winterhalter, Charles;
Schramm, Frederic D;
Pintar, Sara;
... Murray, Heath; + view all
(2023)
The bacterial replication origin BUS promotes nucleobase capture.
NATURE COMMUNICATIONS
, 14
(1)
, Article 8339. 10.1038/s41467-023-43823-w.
Preview |
Text
The bacterial replication origin BUS promotes nucleobase capture.pdf - Accepted Version Download (5MB) | Preview |
Abstract
Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated by the ubiquitous DnaA protein, which assembles into an oligomeric complex at the chromosome origin (oriC) that engages both double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) to promote DNA duplex opening. However, the mechanism of DnaA specifically opening a replication origin was unknown. Here we show that Bacillus subtilis DnaAATP assembles into a continuous oligomer at the site of DNA melting, extending from a dsDNA anchor to engage a single DNA strand. Within this complex, two nucleobases of each ssDNA binding motif (DnaA-trio) are captured within a dinucleotide binding pocket created by adjacent DnaA proteins. These results provide a molecular basis for DnaA specifically engaging the conserved sequence elements within the bacterial chromosome origin basal unwinding system (BUS).
| Type: | Article |
|---|---|
| Title: | The bacterial replication origin BUS promotes nucleobase capture |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41467-023-43823-w |
| Publisher version: | http://dx.doi.org/10.1038/s41467-023-43823-w |
| Language: | English |
| Additional information: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| Keywords: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, SINGLE-STRANDED-DNA, STRUCTURAL BASIS, ESCHERICHIA-COLI, CRYO-EM, CHROMOSOMAL REPLICATION, INITIATION COMPLEX, PROTEIN, RECOGNITION, REFINEMENT, PREDICTION |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10191049 |
Archive Staff Only
 |
View Item |
