Neufeldt, Fiona Catrin Mareile Charlotte;
(2024)
Modelling and Correction of Distortions
and Aberrations in Acousto-Optic Lens
Scanning Microscopy.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
At present, the two-photon (2P) laser scanning fluorescence microscope is one of the most effective imaging techniques available for the study of neuronal function and information processing in the brain. Reasons for its popularity over previous optical methods include an enhanced imaging resolution, signal to noise ratio and resistance to depth-dependent tissue scattering effects. However, image acquisition in 2P microscopy requires that the fluorescence excitation spot be scanned across the 3D field of view. Given the high speed of neuronal dynamics, this demands a very high laser scan speed – one which cannot be fulfilled by combining traditional mechanical scan and refocus mechanisms. To address this limitation, the last couple of decades has seen the emergence of a variety of remote focus (RF) techniques, which achieve low-inertia refocusing by phase modulation of the laser beam. Within the family of RF devices, acousto-optic lenses (AOLs) are unique in their ability to selectively sample the regions of interest at particularly high speed, as has been demonstrated using the 2P AOL microscope developed within the Silver Lab at UCL. However, the scan precision and imaging performance of the AOL microscope and RF microscopes in general, are prone to suffer from the effects of particular optical aberrations and distortions that occur as a by-product of remote focusing. The research described in this thesis focuses on the correction of these RF distortions and aberrations. A ray model of the AOL microscope is developed for the validation of a 3D RF distortion precompensation scheme and to identify the source of an irregular AOL-specific distortion, from which follows a correction of this distortion and the restoration of a sub-micron scan precision. Aberrations affecting the performance of the AOL microscope are identified and characterised by modelling, leading to the first experimental demonstration of partial 3D spherical aberration correction by the AOL.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | Modelling and Correction of Distortions and Aberrations in Acousto-Optic Lens Scanning Microscopy |
| Language: | English |
| Additional information: | Copyright © The Author 2023. Original content in this thesis is licensed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) Licence (https://creativecommons.org/licenses/by/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Electronic and Electrical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10189269 |
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