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Surface Corrosion from Implant–Abutment Couplings with Different Connection Designs Influences Osteoblasts’ Function: A Novel Technique

Alrabeah, Ghada; Knowles, Jonathan C; Petridis, Haralampos; (2023) Surface Corrosion from Implant–Abutment Couplings with Different Connection Designs Influences Osteoblasts’ Function: A Novel Technique. Applied Sciences , 13 (15) , Article 8957. 10.3390/app13158957. Green open access

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Abstract

The improved peri-implant bone response demonstrated when utilizing the platform-switching concept may result from the reduced levels of metal ions released from implant–abutment surfaces to the surrounding tissues. These corrosion products may play a major role in crestal bone remodeling around dental implants. This study evaluated the effect of different implant–abutment couplings (platform-matched vs. platform-switched) on osteoblasts’ function. Titanium alloy and cobalt–chrome alloy abutments were coupled with titanium cylinders, forming either platform-switched or platform-matched groups, and were incubated in human osteoblast cultures utilizing a novel direct-exposure technique. Viability was evaluated over 21 days using Alamar Blue assay. Apoptosis was measured after 24 h using flow cytometry. The expression of genes related to bone resorption was analysed over 21 days using a real-time quantitative polymerase chain reaction assay. Cell viability was reduced from day 4 to day 21 (p < 0.05), with higher rates of early apoptosis (p < 0.05) compared to the controls. Apoptosis was higher in the platform-matched groups (p < 0.05). The tested genes’ expression was up-regulated after 1 and 3 days of exposure to implant–abutment couplings (p < 0.05). The upregulation was more pronounced in platform-matched groups (p < 0.05). Exposure of osteoblasts to implant–abutment couplings induced adverse biological responses, which were more pronounced with platform-matched couplings. These reactions might be related to the increased amounts of metal ions released from the platform-matched couplings, highlighting the possible role of corrosion products in the mediation of crestal bone loss around dental implants.

Type: Article
Title: Surface Corrosion from Implant–Abutment Couplings with Different Connection Designs Influences Osteoblasts’ Function: A Novel Technique
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/app13158957
Publisher version: https://doi.org/10.3390/app13158957
Language: English
Additional information: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/)
Keywords: surface corrosion; dental implants; peri-implantitis; alveolar bone loss; platform-switching; titanium; metal ions; osteoblasts
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Restorative Dental Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10174781
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