Foylan, Shannan;
Schniete, Jana Katharina;
Kölln, Lisa Sophie;
Dempster, John;
Hansen, Carsten Gram;
Shaw, Michael;
Bushell, Trevor John;
(2023)
Mesoscale standing wave imaging.
Journal of Microscopy
10.1111/jmi.13189.
(In press).
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Abstract
Standing wave (SW) microscopy is a method that uses an interference pattern to excite fluorescence from labelled cellular structures and produces high-resolution images of three-dimensional objects in a two-dimensional dataset. SW microscopy is performed with high-magnification, high-numerical aperture objective lenses, and while this results in high-resolution images, the field of view is very small. Here we report upscaling of this interference imaging method from the microscale to the mesoscale using the Mesolens, which has the unusual combination of a low-magnification and high-numerical aperture. With this method, we produce SW images within a field of view of 4.4 mm × 3.0 mm that can readily accommodate over 16,000 cells in a single dataset. We demonstrate the method using both single-wavelength excitation and the multi-wavelength SW method TartanSW. We show application of the method for imaging of fixed and living cells specimens, with the first application of SW imaging to study cells under flow conditions.
| Type: | Article |
|---|---|
| Title: | Mesoscale standing wave imaging |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/jmi.13189 |
| Publisher version: | https://doi.org/10.1111/jmi.13189 |
| Language: | English |
| Additional information: | © 2023 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | fluorescence, interference, mesoscopy, microscopy, standing wave, topography |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10170073 |
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