Ambrose-Dempster, Esther;
Leipold, Leona;
Dobrijevic, Dragana;
Bawn, Maria;
Carter, Eve M;
Stojanovski, Gorjan;
Sheppard, Tom D;
... Hailes, Helen C; + view all
(2023)
Mechanoenzymatic reactions for the hydrolysis of PET.
RSC Advances
, 13
(15)
pp. 9954-9962.
10.1039/d3ra01708g.
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Abstract
Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer into its monomers for recycling or other uses. Mechanoenzymatic reactions have gained traction recently as a green and efficient alternative to traditional biocatalytic reactions. For the first time we report increased yields of PET degradation by whole cell PETase enzymes by up to 27-fold by utilising ball milling cycles of reactive aging, when compared with typical solution-based reactions. This methodology leads to up to a 2600-fold decrease in the solvent required when compared with other leading degradation reactions in the field and a 30-fold decrease in comparison to reported industrial scale PET hydrolysis reactions.
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