Hughes, Tessa;
Dijkstra, Krijn K;
Rawlins, Emma L;
Hynds, Robert E;
(2023)
Open questions in human lung organoid research.
Frontiers in Pharmacology
, 13
, Article 1083017. 10.3389/fphar.2022.1083017.
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Abstract
Organoids have become a prominent model system in pulmonary research. The ability to establish organoid cultures directly from patient tissue has expanded the repertoire of physiologically relevant preclinical model systems. In addition to their derivation from adult lung stem/progenitor cells, lung organoids can be derived from fetal tissue or induced pluripotent stem cells to fill a critical gap in modelling pulmonary development in vitro. Recent years have seen important progress in the characterisation and refinement of organoid culture systems. Here, we address several open questions in the field, including how closely organoids recapitulate the tissue of origin, how well organoids recapitulate patient cohorts, and how well organoids capture diversity within a patient. We advocate deeper characterisation of models using single cell technologies, generation of more diverse organoid biobanks and further standardisation of culture media.
| Type: | Article |
|---|---|
| Title: | Open questions in human lung organoid research |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fphar.2022.1083017 |
| Publisher version: | https://doi.org/10.3389/fphar.2022.1083017 |
| Language: | English |
| Additional information: | © 2023 Hughes, Dijkstra, Rawlins and Hynds. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Keywords: | Lung stem cells, respiratory biology, 3D cell culture, in vitro models, epithelial cells |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10164008 |
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