Riahi (née Bharath), Anouska;
(2022)
Novelty processing and smart delivery of Ganoderma Lucidum spores.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
In recent decades the traditional Chinese medicinal mushroom Ganoderma lucidum (GL), a fungal specie widely consumed homoeopathically in the Eastern Hemisphere, has been studied particularly with respect to antitumour and immunoenhancing effects. Research into the various claims however remains limited owing to the lack of quality and consistency across investigations. As such, efficacy and feasibility of scaleup has not been evaluated in a way that allows widespread consumption or approved treatment. This project tackles three aspects of drug development from Ganoderma lucidum: Biocompound extraction, healthcare evaluation via in-vitro testing, and encapsulation for smart delivery. These avenues are brought together for the first time to evaluate the prospects of developing GL for effective and safe healthcare. This research investigates the parameters that would influence the extractability of a biocompound from the spores of Ganoderma lucidum (GLS), via two conventional methods: Hot Water Extraction (HWE) and Ultrasound-Assisted Extraction (UAE). They are evaluated with respect to their crude water-soluble polysaccharide yield (GLPS). Solvent polarity and process duration were statistically significant factors affecting extract yield, with both extraction methods showing considerable gains over similar setups in literature, recovering over 6% crude GLPS using shorter durations and lower temperatures than other published investigations. This investigation highlighted the importance of solvent viscosity on specific DGlucan extraction in the GLPS yield. Bioactive effects of the extract were evaluated via cytotoxicity toward Human Osteosarcoma (HOS) cells in-vitro, achieving over 40% cell growth inhibition. Cytotoxicity however was only achieved when water-insoluble fractions were administered – suggesting cytotoxicity was a result of the unextracted crude triterpenoids (GLTP) containing Ganoderic Acids. Therefore, HOS-inhibitory capabilities are then compared to a GLPS extract containing Ganoderic Acids (in this work termed “PSGA”), extracted using HWE subject to supervised machine learning optimisation. As well as determining that this yield was maximised at the longest HWE duration and smallest solvent volume, it was observed to inhibit HOS growth by nearly 58% after 24 hours. Low doses and shorter incubation were most effective - suggesting concepts such as resistance (clonal selectivity) and delayed apoptosis, but further work will verify the reported effects of PSGA dosage and exposure time on cancer proliferation. Lastly, research effort is devoted to creating an alginate matrix for the controllable delivery of GLS using Electrohydrodynamic Atomisation (EHDA). Significant effects of the system’s process parameters on particle morphology are observed, in particular EHDA voltage. The carrier’s size, shape and surface features are correlated with its release profile. Importantly, GLS content (something traditionally compromised to maintain particle integrity) was maximised at 50 wt% whilst maintaining a controlled and spherical shape and size – making this study novel and extremely important. It is established that GLS-Alginate particles could offer controlled release over a 2-week administration in pH-neutral conditions; an environment not yet established as “stable” for alginate, yet reflective of physiological passage. Thus, for the first time sodium alginate is proven to be a real contender in controlling the delivery of GLS biomolecules. The reconciliation of these essential stages of drug development highlights some crucial points of focus as GL continues to undergo rigorous development in the realm of drug discovery.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | Novelty processing and smart delivery of Ganoderma Lucidum spores |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Copyright © The Author 2023. Original content in this thesis is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Licence (https://creativecommons.org/licenses/by-nc/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10162612 |
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