Critchley, William R;
Fearnley, Gareth WF;
Abdul-Zani, Izma;
Molina-Paris, Carmen;
Bendtsen, Claus;
Zachary, Ian C;
Harrison, Michael A;
(2022)
Monitoring VEGF-Stimulated Calcium Ion Flux in Endothelial Cells.
Methods in Molecular Biology
, 2475
pp. 113-124.
10.1007/978-1-0716-2217-9_7.
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Abstract
The endothelial response to vascular endothelial growth factor A (VEGF-A) regulates many aspects of animal physiology in health and disease. Such VEGF-A-regulated phenomena include vasculogenesis, angiogenesis, tumor growth and progression. VEGF-A binding to receptor tyrosine kinases such as vascular endothelial growth factor receptor 2 (VEGFR2 ) activates multiple signal transduction pathways and changes in homeostasis, metabolism, gene expression, cell proliferation, migration, and survival. One such VEGF-A-regulated response is a rapid rise in cytosolic calcium ion levels which modulates different biochemical events and impacts on endothelial-specific responses. Here, we present a series of detailed and robust protocols for evaluating ligand-stimulated cytosolic calcium ion flux in endothelial cells. By monitoring an endogenous endothelial transcription factor (NFATc2 ) which displays calcium-sensitive redistribution, we can assess the relevance of cytosolic calcium to protein function. This protocol can be easily applied to both adherent and non-adherent cultured cells to evaluate calcium ion flux in response to exogenous stimuli such as VEGF-A.
| Type: | Article |
|---|---|
| Title: | Monitoring VEGF-Stimulated Calcium Ion Flux in Endothelial Cells |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-2217-9_7 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-2217-9_7 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. |
| Keywords: | Endothelial cells, Calcium, VEGF-A, VEGFR2, NFATc2, Human umbilical vein endothelial cells (HUVECs) |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Experimental and Translational Medicine |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10160041 |
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