Xie, Yi;
Li, Huimin;
Chen, Fumin;
Udayakumar, Srisruthi;
Arora, Khyati;
Chen, Hui;
Lan, Yang;
... Yin, Kun; + view all
(2022)
Clustered Regularly Interspaced short palindromic repeats-Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives.
Advanced Science
, Article e2204172. 10.1002/advs.202204172.
(In press).
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Abstract
Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. Conventional diagnostic techniques for infectious diseases typically require sophisticated equipment and are time consuming. Emerging clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) detection systems have shown remarkable potential as next-generation diagnostic tools to achieve rapid, sensitive, specific, and field-deployable diagnoses of infectious diseases, based on state-of-the-art microfluidic platforms. Therefore, a review of recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis is urgently required. This review highlights the mechanisms of CRISPR/Cas biosensing and cutting-edge microfluidic devices including paper, digital, and integrated wearable platforms. Strategies to simplify sample pretreatment, improve diagnostic performance, and achieve integrated detection are discussed. Current challenges and future perspectives contributing to the development of more effective CRISPR-based microfluidic diagnostic systems are also proposed.
| Type: | Article |
|---|---|
| Title: | Clustered Regularly Interspaced short palindromic repeats-Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/advs.202204172 |
| Publisher version: | https://doi.org/10.1002/advs.202204172 |
| Language: | English |
| Additional information: | © 2022 The Authors. Advanced Science published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats-associated proteins biosensing mechanisms, infectious diseases diagnosis, integrated detection, microfluidic platforms |
| UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10157905 |
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Loading...Loading...| 1. |  China | 12 |
| 2. |  United States | 10 |
| 3. |  Russian Federation | 4 |
| 4. |  India | 3 |
| 5. |  United Kingdom | 2 |
| 6. |  Germany | 2 |
| 7. |  Japan | 1 |
| 8. |  Czech Republic | 1 |
| 9. |  France | 1 |
| 10. |  Italy | 1 |
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