Nezhentsev, Andrey;
Abhari, Roxanna E;
Baldwin, Mathew J;
Mimpen, Jolet Y;
Augustyniak, Edyta;
Isaacs, Mark;
Mouthuy, Pierre-Alexis;
... Snelling, Sarah JB; + view all
(2021)
In vitro evaluation of the response of human tendon-derived stromal cells to a novel electrospun suture for tendon repair.
Translational Sports Medicine
, 4
(3)
pp. 409-418.
10.1002/tsm2.233.
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Abstract
Recurrent tears after surgical tendon repair remain common. Repair failures can be partly attributed to the use of sutures not designed for the tendon cellular niche nor for the promotion of repair processes. Synthetic electrospun materials can mechanically support the tendon whilst providing topographical cues that regulate cell behaviour. Here, a novel electrospun suture made from twisted polydioxanone (PDO) polymer filaments is compared to PDS II, a clinically-used PDO suture currently utilised in tendon repair. We evaluated the ability of these sutures to support the attachment and proliferation of human tendon-derived stromal cells using PrestoBlue and Scanning Electron Microscopy. Suture surface chemistry was analysed using X-ray Photoelectron Spectroscopy. Bulk RNA-Seq interrogated the transcriptional response of primary tendon-derived stromal cells to sutures after 14 days. Electrospun suture showed increased initial cell attachment and a stronger transcriptional response compared to PDS II, with relative enrichment of pathways including mTorc1 signalling and depletion of epithelial mesenchymal transition. Neither suture induced transcriptional upregulation of inflammatory pathways compared to baseline. Twisted electrospun sutures therefore show promise in improving outcomes in surgical tendon repair by allowing increased cell attachment whilst maintaining an appropriate tissue response.
| Type: | Article |
|---|---|
| Title: | In vitro evaluation of the response of human tendon-derived stromal cells to a novel electrospun suture for tendon repair |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/tsm2.233 |
| Publisher version: | https://doi.org/10.1002/tsm2.233 |
| Language: | English |
| Additional information: | © 2021 The Authors. Translational Sports Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Electrospinning, Electrospun suture, PDS II, Polydioxanone, Tendon repair, Tissue engineering |
| UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry UCL > Provost and Vice Provost Offices > UCL BEAMS UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10157181 |
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