Sampson, O;
Lim, N;
White, J;
Vieira, V;
Kløverpris, H;
Adland, E;
Conlon, C;
... Goulder, P; + view all
(2022)
A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells.
Journal of Immunological Methods
, 504
, Article 113263. 10.1016/j.jim.2022.113263.
Preview |
Text
1-s2.0-S0022175922000503-main.pdf - Other Download (4MB) | Preview |
Abstract
Central to sex differences observed in outcome from infection and vaccination is the innate immune response, and specifically production of type I interferons by plasmacytoid dendtiric cells (pDCs), the main producers of IFN-α. Evaluation of IFN-α production by pDCs is therefore critical for studies of innate immune function. However, reliable measurement of pDC IFN-α is hampered by reduced cell yields and cytokine production after cryopreservation or after even short delays in stimulating freshly isolated cells. We here describe a simple yet robust method for measuring IFN-α production in pDCs that preserves cell activation and cytokine production through immediate stimulation of whole blood and subsequent maintenance at 37 °C.
Type: | Article |
---|---|
Title: | A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells |
Location: | Netherlands |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1016/j.jim.2022.113263 |
Publisher version: | https://doi.org/10.1016/j.jim.2022.113263 |
Language: | English |
Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
Keywords: | Immune sex differences, Innate immunity, Interferon alpha, Plasmacytoid dendritic cell, Toll-like receptor 7, Dendritic Cells, Female, Flow Cytometry, Humans, Immunity, Innate, Interferon Type I, Interferon-alpha, Male |
UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL |
URI: | https://discovery.ucl.ac.uk/id/eprint/10151754 |



1. | ![]() | 8 |
2. | ![]() | 3 |
3. | ![]() | 1 |
4. | ![]() | 1 |
5. | ![]() | 1 |
6. | ![]() | 1 |
Archive Staff Only
![]() |
View Item |