Plazonic, Filip;
LuTheryn, Gareth;
Hind, Charlotte;
Clifford, Melanie;
Gray, Michael;
Stride, Eleanor;
Glynne-Jones, Peter;
... Carugo, Dario; + view all
(2022)
Bactericidal Effect of Ultrasound-Responsive Microbubbles and Sub-inhibitory Gentamicin against Pseudomonas aeruginosa Biofilms on Substrates With Differing Acoustic Impedance.
Ultrasound in Medicine & Biology
10.1016/j.ultrasmedbio.2022.05.019.
(In press).
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Abstract
The aim of this research was to explore the interaction between ultrasound-activated microbubbles (MBs) and Pseudomonas aeruginosa biofilms, specifically the effects of MB concentration, ultrasound exposure and substrate properties on bactericidal efficacy. Biofilms were grown using a Centre for Disease Control (CDC) bioreactor on polypropylene or stainless-steel coupons as acoustic analogues for soft and hard tissue, respectively. Biofilms were treated with different concentrations of phospholipid-shelled MBs (107 108 MB/mL), a sub-inhibitory concentration of gentamicin (4 mg/mL) and 1-MHz ultrasound with a continuous or pulsed (100-kHz pulse repetition frequency, 25% duty cycle, 0.5-MPa peak-to-peak pressure) wave. The effect of repeated ultrasound exposure with intervals of either 15- or 60-min was also investigated. With polypropylene coupons, the greatest bactericidal effect was achieved with 2 £ 5 min of pulsed ultrasound separated by 60 min and a microbubble concentration of 5 £ 107 MBs/mL. A 0.76 log (83%) additional reduction in the number of bacteria was achieved compared with the use of an antibiotic alone. With stainless-steel coupons, a 67% (0.46 log) reduction was obtained under the same exposure conditions, possibly due to enhancement of a standing wave field which inhibited MB penetration in the biofilm. These findings demonstrate the importance of treatment parameter selection in antimicrobial applications of MBs and ultrasound in different tissue environments.
| Type: | Article |
|---|---|
| Title: | Bactericidal Effect of Ultrasound-Responsive Microbubbles and Sub-inhibitory Gentamicin against Pseudomonas aeruginosa Biofilms on Substrates With Differing Acoustic Impedance |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ultrasmedbio.2022.05.019 |
| Publisher version: | https://doi.org/10.1016/j.ultrasmedbio.2022.05.019 |
| Language: | English |
| Additional information: | Copyright © 2022 The Author(s). Published by Elsevier Inc. on behalf of World Federation for Ultrasound in Medicine & Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) Printed in the USA. All rights reserved. |
| Keywords: | Ultrasound, Biofilm, Bacteria, Microbubble, Antibiotic, Antimicrobial resistance, Chronic wound, Substrate. |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10151247 |
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