Guha, Arnab;
Sandström, Niklas;
Ostanin, Victor P;
Klenerman, David;
Ghosh, Sourav K;
(2022)
Simpler and faster quartz crystal microbalance for macromolecule detection using Fixed Frequency Drive.
Sensors and Actuators B: Chemical
, 358
, Article 131442. 10.1016/j.snb.2022.131442.
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Abstract
Despite advancements in analytical technologies, their complexity and cost have largely restricted their application in scalable online or multiplexed measurements. Here we report a quartz crystal resonator (QCR)-based method for detection of macromolecules that allows immensely simpler and faster measurements by employing for the first time a fixed frequency drive (FFD) and analytical expressions of acoustic parameters. Using human immunoglobulin E (hIgE) as an exemplar macromolecule and an anti-hIgE aptamer functionalised on a QCR, quantitative accuracy was benchmarked against the traditional impedance analysis method. The ability of FFD to capture data over longer observation periods at significantly higher acquisition rates at a fixed amplitude showed improvement in the QCR’s sensitivity and specificity of transduction. The foundations for low-cost and low-power online integration and large-scale multiplexability are also discussed.
| Type: | Article |
|---|---|
| Title: | Simpler and faster quartz crystal microbalance for macromolecule detection using Fixed Frequency Drive |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.snb.2022.131442 |
| Publisher version: | https://doi.org/10.1016/j.snb.2022.131442 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Quartz crystal microbalance (QCM), Aptamer, Human immunoglobulin E (hIgE), Fixed frequency drive (FFD), Realtime aptasensor, Simple immunosensor, Immunoprotein detection |
| UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10143148 |
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