Andreassi, C;
Riccio, A;
(2021)
A detailed protocol for RNA cleavage assay in sympathetic neurons.
STAR Protocols
, 2
(4)
, Article 101001. 10.1016/j.xpro.2021.101001.
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Abstract
This protocol illustrates the use of an in vitro assay to study the cleavage of the IMPA1 3′UTR by the endonuclease Ago2 in sympathetic neurons. The procedure includes the preparation of cytoplasmic protein extracts and also describes the synthesis and labeling of the RNA probe. The protocol can be applied to other cell systems, RNA transcripts, and endonucleases to confirm the role of known cleavage site(s) and cleavage proteins, or to investigate new ones. For complete details on the use and execution of this protocol, please refer to Andreassi et al. (2021).
| Type: | Article |
|---|---|
| Title: | A detailed protocol for RNA cleavage assay in sympathetic neurons |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xpro.2021.101001 |
| Publisher version: | https://doi.org/10.1016/j.xpro.2021.101001 |
| Language: | English |
| Additional information: | © 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10140847 |
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