Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis

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Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis

Slamova, I; Adib, R; Ellmerich, S; Golos, MR; Gilbertson, JA; Botcher, N; Canetti, D; ... Simons, JP; + view all (2021) Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis. Nature Communications , 12 (1) , Article 7112. 10.1038/s41467-021-27416-z. Green open access

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Abstract

Cardiac ATTR amyloidosis, a serious but much under-diagnosed form of cardiomyopathy, is caused by deposition of amyloid fibrils derived from the plasma protein transthyretin (TTR), but its pathogenesis is poorly understood and informative in vivo models have proved elusive. Here we report the generation of a mouse model of cardiac ATTR amyloidosis with transgenic expression of human TTRS52P. The model is characterised by substantial ATTR amyloid deposits in the heart and tongue. The amyloid fibrils contain both full-length human TTR protomers and the residue 49-127 cleavage fragment which are present in ATTR amyloidosis patients. Urokinase-type plasminogen activator (uPA) and plasmin are abundant within the cardiac and lingual amyloid deposits, which contain marked serine protease activity; knockout of α2-antiplasmin, the physiological inhibitor of plasmin, enhances amyloid formation. Together, these findings indicate that cardiac ATTR amyloid deposition involves local uPA-mediated generation of plasmin and cleavage of TTR, consistent with the previously described mechano-enzymatic hypothesis for cardiac ATTR amyloid formation. This experimental model of ATTR cardiomyopathy has potential to allow further investigations of the factors that influence human ATTR amyloid deposition and the development of new treatments.

Type:	Article
Title:	Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis
Location:	England
Open access status:	An open access version is available from UCL Discovery
DOI:	10.1038/s41467-021-27416-z
Publisher version:	http://doi.org/10.1038/s41467-021-27416-z
Language:	English
Additional information:	This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Keywords:	Cardiovascular biology, Experimental models of disease, Molecular medicine, Protein folding, Proteolysis
UCL classification:	UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI:	https://discovery.ucl.ac.uk/id/eprint/10140321