Rashidi, H;
Hay, DC;
(2021)
Serum-Free Production of Three-Dimensional Hepatospheres from Pluripotent Stem Cells.
Methods in Molecular Biology
, 2454
pp. 305-316.
10.1007/7651_2021_430.
Preview |
Text
Rashidi_Serum-Free Production of Three-Dimensional Hepatospheres from Pluripotent Stem Cells_AAM.pdf - Accepted Version Download (616kB) | Preview |
Abstract
Developing renewable human liver tissue from stem cells has been pursued as a potential source of biological material for pharmaceutical and clinical endeavors. At present, two-dimensional differentiation procedures deliver tissue lacking long-term phenotypic and functional stability. Efforts to overcome these limiting factors have led to the development of protocols to generate three-dimensional cellular aggregates. Here we describe a methodology to generate 3D hepatospheres from human pluripotent stem cells using defined and commercially available reagents.
| Type: | Article |
|---|---|
| Title: | Serum-Free Production of Three-Dimensional Hepatospheres from Pluripotent Stem Cells |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/7651_2021_430 |
| Publisher version: | https://doi.org/10.1007/7651_2021_430 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. |
| Keywords: | 3D cell culture, Hepatic differentiation, Human pluripotent stem cells, Parenchymal cells, Regenerative Medicine |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10139914 |
Archive Staff Only
 |
View Item |
