Benn, G;
Mikheyeva, IV;
Inns, PG;
Forster, JC;
Ojkic, N;
Bortolini, C;
Ryadnov, MG;
... Hoogenboom, BW; + view all
(2021)
Phase separation in the outer membrane of Escherichia coli.
Proceedings of the National Academy of Sciences
, 118
(44)
, Article e2112237118. 10.1073/pnas.2112237118.
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Abstract
Antimicrobial resistance is particularly prevalent in gram-negative bacteria, as antibiotics that act inside the cells must overcome their outer membrane. So far, technical limitations have prevented us from determining how outer-membrane proteins and lipids are organized to form this functional barrier. Here, we use nanoscale imaging of live bacteria to reveal that the most abundant outer-membrane proteins form a network that spans the entire bacterial surface, leaving only small gaps of phase-separated lipopolysaccharide. This tendency to phase separate is further emphasized by the formation of new domains when phospholipids are mislocated at the surface, rendering cells more susceptible to some antibiotics. Overall, the phase-separated nature of the outer membrane defines a perspective on its integrity and barrier function.
| Type: | Article |
|---|---|
| Title: | Phase separation in the outer membrane of Escherichia coli |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1073/pnas.2112237118 |
| Publisher version: | https://doi.org/10.1073/pnas.2112237118 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. |
| Keywords: | atomic force microscopy, gram-negative bacteria, outer membrane, phase separation |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10138015 |
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