Baumann, E;
Pohle, U;
Zhang, E;
Allen, T;
Villringer, C;
Pulwer, S;
Gerhardt, H;
(2021)
A backward-mode optical-resolution photoacoustic microscope for 3D imaging using a planar Fabry-Pérot sensor.
Photoacoustics
, 24
, Article 100293. 10.1016/j.pacs.2021.100293.
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Abstract
Optical-resolution photoacoustic microscopy (OR-PAM) combines high spatial resolution and strong absorption-based contrast in tissue, which has enabled structural and spectroscopic imaging of endogenous chromophores, primarily hemoglobin. Conventional piezoelectric ultrasound transducers are typically placed far away from the photoacoustic source due to their opacity, which reduces acoustic sensitivity. Optical ultrasound sensors are an alternative as their transparency allows them to be positioned close to the sample with minimal source-detector distances. In this work, a backward-mode OR-PAM system based on a planar Fabry-Pérot ultrasound sensor and coaxially aligned excitation and interrogation beams was developed. Two 3D imaging modes, using raster-scanning for enhanced image quality and continuous-scanning for fast imaging, were implemented and tested on a leaf skeleton phantom. In fast imaging mode, a scan-rate of 100,000 A-lines/s was achieved. 3D images of a zebrafish embryo were acquired in vivo in raster-scanning mode. The transparency of the FP sensor in the visible and near-infrared wavelength region makes it suitable for combined functional and molecular imaging applications using OR-PAM and multi-photon fluorescence microscopy.
| Type: | Article |
|---|---|
| Title: | A backward-mode optical-resolution photoacoustic microscope for 3D imaging using a planar Fabry-Pérot sensor |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.pacs.2021.100293 |
| Publisher version: | https://doi.org/10.1016/j.pacs.2021.100293 |
| Language: | English |
| Additional information: | © 2021 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Optical-resolution photoacoustic microscopy, Backward-mode imaging, Dual-wavelength, Continuous scanning, In vivo imaging, Optical ultrasound sensing, Planar Fabry-Pérot interferometer |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10133863 |
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