Blackie, L; Tozluoglu, M; Trylinski, M; Walther, RF; Schweisguth, F; Mao, Y; Pichaud, F; (2021) A combination of notch signaling, preferential adhesion and endocytosis induces a slow mode of cell intercalation in the Drosophila retina. Development 10.1242/dev.197301. (In press).

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Abstract

Movement of epithelial cells in a tissue occurs through neighbor exchange and drives tissue shape changes. It requires intercellular junction remodeling, a process typically powered by the contractile actomyosin cytoskeleton. This has mostly been investigated in homogeneous epithelia where intercalation takes minutes. However, in some tissues, intercalation involves different cell types and can take hours. Whether slow and fast intercalation share the same mechanisms remains to be examined. To address this issue, we use the fly eye, where the cone cells exchange neighbors over approximately 10 hours to shape the lens. We uncover three pathways regulating this slow mode of cell intercalation. Firstly, we find a limited requirement for MyosinII. In this case, mathematical modeling predicts an adhesion dominant intercalation mechanism. Genetic experiments support this prediction and reveal a role for adhesion through the Nephrin proteins Roughest and Hibris. Secondly, we find cone cell intercalation is regulated by the Notch-signaling pathway. Thirdly, we show endocytosis is required for membrane removal and Notch activation. Altogether, our work indicates that adhesion, endocytosis and Notch can induce junction remodeling over long-time scales.

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