Genovese, G;
Palombo, M;
Santin, MD;
Valette, J;
Ligneul, C;
Aigrot, M-S;
Abdoulkader, N;
... Branzoli, F; + view all
(2021)
Inflammation‐driven glial alterations in the cuprizone mouse model probed with diffusion‐weighted magnetic resonance spectroscopy at 11.7 T.
NMR in Biomedicine
, 34
(4)
, Article e4480. 10.1002/nbm.4480.
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Abstract
Inflammation of brain tissue is a complex response of the immune system to the presence of toxic compounds or to cell injury, leading to a cascade of pathological processes that include glial cell activation. Noninvasive MRI markers of glial reactivity would be very useful for in vivo detection and monitoring of inflammation processes in the brain, as well as for evaluating the efficacy of personalized treatments. Due to their specific location in glial cells, myo‐inositol (mIns) and choline compounds (tCho) seem to be the best candidates for probing glial‐specific intra‐cellular compartments. However, their concentrations quantified using conventional proton MRS are not specific for inflammation. In contrast, it has been recently suggested that mIns intra‐cellular diffusion, measured using diffusion‐weighted MRS (DW‐MRS) in a mouse model of reactive astrocytes, could be a specific marker of astrocytic hypertrophy. In order to evaluate the specificity of both mIns and tCho diffusion to inflammation‐driven glial alterations, we performed DW‐MRS in a volume of interest containing the corpus callosum and surrounding tissue of cuprizone‐fed mice after 6 weeks of intoxication, and evaluated the extent of astrocytic and microglial alterations using immunohistochemistry. Both mIns and tCho apparent diffusion coefficients were significantly elevated in cuprizone‐fed mice compared with control mice, and histologic evaluation confirmed the presence of severe inflammation. Additionally, mIns and tCho diffusion showed, respectively, strong and moderate correlations with histological measures of astrocytic and microglial area fractions, confirming DW‐MRS as a promising tool for specific detection of glial changes under pathological conditions.
| Type: | Article |
|---|---|
| Title: | Inflammation‐driven glial alterations in the cuprizone mouse model probed with diffusion‐weighted magnetic resonance spectroscopy at 11.7 T |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/nbm.4480 |
| Publisher version: | https://doi.org/10.1002/nbm.4480 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | choline compounds, cuprizone model, diffusion-weighted spectroscopy, glial cells, inflammation, myo-inositol |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10126905 |
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