Yue, X;
Bao, M;
Christiano, R;
Li, S;
Mei, J;
Zhu, L;
Mao, F;
... Lee, I; + view all
(2017)
ACBD3 functions as a scaffold to organize the Golgi stacking proteins and a Rab33b-GAP.
FEBS Letters
, 591
(18)
pp. 2793-2802.
10.1002/1873-3468.12780.
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Abstract
Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial‐Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl‐CoA binding domain containing 3 (ACBD3) is likely to be a novel binding partner of Golgin45. ACBD3 interacts with Golgin45 via its GOLD domain, while its co‐expression significantly increases Golgin45 targeting to the Golgi. Furthermore, ACBD3 recruits TBC1D22, a Rab33b GTPase activating protein (GAP), to a large multi‐protein complex containing Golgin45 and GRASP55. These results suggest that ACBD3 may provide a scaffolding to organize the Golgi stacking proteins and a Rab33b‐GAP at the medial‐Golgi.
| Type: | Article |
|---|---|
| Title: | ACBD3 functions as a scaffold to organize the Golgi stacking proteins and a Rab33b-GAP |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/1873-3468.12780 |
| Publisher version: | https://doi.org/10.1002/1873-3468.12780 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, Cell Biology, ACBD3, Golgi, Golgin45, membrane trafficking, Rab-GTPase, CELL-FREE SYSTEM, MATRIX PROTEIN, COMPLEX, GM130, GRASP55, GCP60, IDENTIFICATION, REPLICATION, PROTEOMICS, CISTERNAE |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10126399 |
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