Karunanithy, G;
Shukla, V;
Hansen, DF;
(2021)
Methodological Advancements for Characterising Protein Side Chains by NMR Spectroscopy.
Current Opinion in Structural Biology
, 70
pp. 61-69.
10.1016/j.sbi.2021.04.002.
Preview |
Text
UCL_rps_Deposit.pdf - Accepted Version Download (306kB) | Preview |
Abstract
The surface of proteins is covered by side chains of polar amino acids that are imperative for modulating protein functionality through the formation non-covalent intermolecular interactions. However, despite their tremendous importance, the unique structures of protein side chains require tailored approaches for investigation by NMR spectroscopy, and so have traditionally been understudied compared to the protein backbone. Here, we review substantial recent methodological advancements within NMR spectroscopy to address this issue. Specifically, we consider advancements that provide new insight into methyl-bearing side chains, show the potential of using non-natural amino acids, and reveal the actions of charged side chains. Combined, the new methods promise unprecedented characterisations of side chains that will further elucidate protein function.
| Type: | Article |
|---|---|
| Title: | Methodological Advancements for Characterising Protein Side Chains by NMR Spectroscopy |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.sbi.2021.04.002 |
| Publisher version: | http://dx.doi.org/10.1016/j.sbi.2021.04.002 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | NMR spectroscopy, Method developments, Protein side chains, Conformational Exchange, Interactions |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10126398 |
Loading...
Loading...Loading...Loading...Archive Staff Only
 |
View Item |
