Lee, S-C;
Lee, N-H;
Patel, KD;
Jang, T-S;
Knowles, JC;
Kim, H-W;
Lee, H-H;
(2021)
The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells.
Nanomaterials
, 11
(2)
p. 557.
10.3390/nano11020557.
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Abstract
Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H2O2 : 5~20 µg/mL SeNPs recovered cells damaged by 200 µM H2O2 via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were determined, leading to possible applications for bone treatment.
Type: | Article |
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Title: | The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.3390/nano11020557 |
Publisher version: | http://dx.doi.org/10.3390/nano11020557 |
Language: | English |
Additional information: | Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). |
Keywords: | selenium; nanoparticles; osteogenic differentiation; MC3T3-E1; reactive oxygen species |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng |
URI: | https://discovery.ucl.ac.uk/id/eprint/10123494 |
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