Xavier Lopez-Labrador, F;
Brown, JR;
Fischer, N;
Harvala, H;
Van Boheemen, S;
Cinek, O;
Sayiner, A;
... de Vries, JJC; + view all
(2021)
Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure.
jJournal of Clinical Virology
, 134
, Article 104691. 10.1016/j.jcv.2020.104691.
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Abstract
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.
| Type: | Article |
|---|---|
| Title: | Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.jcv.2020.104691 |
| Publisher version: | http://dx.doi.org/10.1016/j.jcv.2020.104691 |
| Language: | English |
| Additional information: | © 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keywords: | Viral metagenomics, High-throughput sequencing, Next-generation sequencing, Wet lab, Recommendations, INFLUENZA-A VIRUS, EFFICIENT TRANSLATION, PATHOGEN DETECTION, MESSENGER-RNA, DNA, VALIDATION, DIAGNOSIS, DEPLETION |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10123057 |
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