Speakman, JR;
Yamada, Y;
Sagayama, H;
Berman, ESF;
Ainslie, PN;
Andersen, LF;
Anderson, LJ;
... Wong, WW; + view all
(2021)
A standard calculation methodology for human doubly labeled water studies.
Cell Reports Medicine
, 2
(2)
, Article 100203. 10.1016/j.xcrm.2021.100203.
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Abstract
The doubly labeled water (DLW) method measures total energy expenditure (TEE) in free-living subjects. Several equations are used to convert isotopic data into TEE. Using the International Atomic Energy Agency (IAEA) DLW database (5,756 measurements of adults and children), we show considerable variability is introduced by different equations. The estimated rCO2 is sensitive to the dilution space ratio (DSR) of the two isotopes. Based on performance in validation studies, we propose a new equation based on a new estimate of the mean DSR. The DSR is lower at low body masses (<10 kg). Using data for 1,021 babies and infants, we show that the DSR varies non-linearly with body mass between 0 and 10 kg. Using this relationship to predict DSR from weight provides an equation for rCO2 over this size range that agrees well with indirect calorimetry (average difference 0.64%; SD = 12.2%). We propose adoption of these equations in future studies.
| Type: | Article |
|---|---|
| Title: | A standard calculation methodology for human doubly labeled water studies |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xcrm.2021.100203 |
| Publisher version: | https://doi.org/10.1016/j.xcrm.2021.100203 |
| Language: | English |
| Additional information: | Copyright © 2021 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | total energy expenditure, free-living, validation, doubly labeled water |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Population, Policy and Practice Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10122887 |
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