Liu, J; Lambert, H; Zhang, Y-W; Lee, T-C; (2021) Rapid Estimation of Binding Constants for Cucurbit[8]uril Ternary Complexes Using Electrochemistry. Analytical Chemistry 10.1021/acs.analchem.0c04887. (In press).

Preview

Text acs.analchem.0c04887.pdf - Published Version Download (2MB) | Preview

Abstract

upramolecular complexes are of fundamental interests in biomedicines and adaptive materials, and thus facile methods to determine their binding affinity show usefulness in the design of novel drugs and materials. Herein, we report a novel approach to estimate the binding constants K_{G2} of cucurbit[8]uril-methyl viologen-based ternary complexes (CB8-MV^{2+}-G2) using electrochemistry, achieving high precision (±0.03) and practical accuracy (±0.32) in logKG2 and short measurement time (<10 min). In particular, we have uncovered a linear correlation (R^{2} > 0.8) between the reduction potential of CB8-MV^{2+}-G2 ternary complexes and their reported binding constants from isothermal titration calorimetry, which allow a calibration curve to be plotted based on 25 sample complexes. Mechanistic investigation using experimental and computational approaches reveals that this correlation stems from the dynamic host-guest exchange events occurring after the electron transfer step. Binding constants of unknown ternary complexes, where G2 = hydrocarbons, were estimated, illustrating potential applications for sparsely soluble second guests.

Download activity - last month

Export as CSVXMLJSON

Download activity - last 12 months

Export as JSONXMLCSV

Downloads by country - last 12 months

Export as CSVJSONXML

Archive Staff Only

View Item