Adams, SP;
Kricke, S;
Ralph, E;
Gilmour, N;
Gilmour, KC;
(2018)
A comparison of TRECs and flow cytometry for naive T cell quantification.
Clinical and Experimental Immunology
, 191
(2)
pp. 198-202.
10.1111/cei.13062.
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Abstract
Assessment of thymic output by measurement of naive T cells is carried out routinely in clinical diagnostic laboratories, predominantly using flow cytometry with a suitable panel of antibodies. Naive T cell measurements can also be made using molecular analyses to quantify T cell receptor excision circle (TRECs) levels in sorted cells from peripheral blood. In this study we have compared TRECs levels retrospectively with CD45RA+ CD27+ T cells and also with CD45RA+ CD31+ T cells in 134 patient samples at diagnosis or during follow-up. Both panels provide naive T cell measurements that have a strongly positive correlation with TRECs numbers and are suitable for use with enumerating naive T cell levels in a clinical laboratory.
| Type: | Article |
|---|---|
| Title: | A comparison of TRECs and flow cytometry for naive T cell quantification |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/cei.13062 |
| Publisher version: | https://doi.org/10.1111/cei.13062 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | CD31, CD45RA, TRECs, naive T cells, Blood Cells, Flow Cytometry, Follow-Up Studies, Humans, Immunologic Memory, Leukocyte Common Antigens, Molecular Diagnostic Techniques, Platelet Endothelial Cell Adhesion Molecule-1, Receptors, Antigen, T-Cell, Retrospective Studies, T-Lymphocyte Subsets, T-Lymphocytes, Tumor Necrosis Factor Receptor Superfamily, Member 7 |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10118396 |
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