Tempest, N;
Jansen, M;
Baker, A-M;
Hill, CJ;
Hale, M;
Magee, D;
Treanor, D;
... Hapangama, DK; + view all
(2020)
Histological 3D reconstruction and in vivo lineage tracing of the human endometrium.
Journal of Pathology
, 251
(4)
pp. 440-451.
10.1002/path.5478.
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Abstract
Regular menstrual shedding and repair of the endometrial functionalis is unique to humans and higher‐order primates. The current consensus postulates endometrial glands to have a single‐tubular architecture, where multi‐potential stem cells reside in the blind‐ending glandular‐bases. Utilising fixed samples from patients, we have studied the three‐dimensional (3D) micro‐architecture of the human endometrium. We demonstrate that some non‐branching, single, vertical functionalis glands originate from a complex horizontally interconnecting network of basalis glands. The existence of a multipotent endometrial epithelial stem cell capable of regenerating the entire complement of glandular lineages was demonstrated by in vivo lineage tracing, using naturally occurring somatic mitochondrial DNA mutations as clonal markers. Vertical tracking of mutated clones showed that at least one stem‐cell population resides in the basalis glands. These novel findings provide insight into the efficient and scar‐less regenerative potential of the human endometrium.
| Type: | Article |
|---|---|
| Title: | Histological 3D reconstruction and in vivo lineage tracing of the human endometrium |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/path.5478 |
| Publisher version: | https://doi.org/10.1002/path.5478 |
| Language: | English |
| Additional information: | © 2020 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | 3D reconstruction, lineage tracing, endometrium, regeneration, stem cells |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Pathology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10112459 |
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