Sanghani-Kerai, A;
Brown, R;
Lodge, G;
Osagie-Clouard, L;
Coathup, M;
Graney, I;
Skinner, J;
... Blunn, G; + view all
(2020)
The Development of a novel autologous blood glue aiming to improve osseointegration in the bone-implant interface.
Bone and Joint Research
, 9
(7)
pp. 402-411.
10.1302/2046-3758.97.BJR-2019-0073.R3.
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Abstract
Aims: For cementless implants, stability is initially attained by an interference fit into the bone and osteo-integration may be encouraged by coating the implant with bioactive substances. Blood based autologous glue provides an easy, cost-effective way of obtaining high concentrations of growth factors for tissue healing and regeneration with the intention of spraying it onto the implant surface during surgery. The aim of this study was to incorporate nucleated cells from autologous bone marrow (BM) aspirate into gels made from the patient's own blood, and to investigate the effects of incorporating three different concentrations of platelet rich plasma (PRP) on the proliferation and viability of the cells in the gel. Methods: The autologous blood glue (ABG) that constituted 1.25, 2.5, and 5 times concentration PRP were made with and without equal volumes of BM nucleated cells. Proliferation, morphology, and viability of the cells in the glue was measured at days 7 and 14 and compared to cells seeded in fibrin glue. Results: Overall, 2.5 times concentration of PRP in ABG was capable of supporting the maximum growth of cells isolated from the BM aspirate and maintain their characteristics. Irrespective of PRP concentration, cells in ABG had statistically significantly higher viability compared to cells in fibrin glue. Conclusion: In vitro this novel autologous gel is more capable of supporting the growth of cells in its structure for up to 14 days, compared to commercially available fibrin-based sealants, and this difference was statistically significant.
| Type: | Article |
|---|---|
| Title: | The Development of a novel autologous blood glue aiming to improve osseointegration in the bone-implant interface |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1302/2046-3758.97.BJR-2019-0073.R3 |
| Publisher version: | https://doi.org/10.1302/2046-3758.97.BJR-2019-0073... |
| Language: | English |
| Additional information: | © 2020 Author(s) et al. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND 4.0) licence, which permits the copying and redistribution of the work only, and provided the original author and source are credited. See https://creativecommons.org/licenses/by-nc-nd/4.0/. |
| Keywords: | Blood glue, Autologous,osteointegration, stem cells, Platelet Rich Plasma |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10110798 |
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