Yang, Y;
Zhang, Z;
Lu, X;
Gu, J;
Wang, Y;
Yao, Y;
Liao, X;
... Hao, J; + view all
(2020)
Production of 2,3-dihydroxyisovalerate by Enterobacter cloacae.
Enzyme and Microbial Technology
, 140
, Article 109650. 10.1016/j.enzmictec.2020.109650.
Preview |
Text (Article)
Baganz_Production of 2,3-dihydroxyisovalerate by E cloacae_accepted MS.pdf - Accepted Version Download (1MB) | Preview |
Preview |
Text (Supplementary data)
Baganz_EC MS_Supplementary data.pdf - Accepted Version Download (249kB) | Preview |
Abstract
2,3-Dihydroxyisovalerate is an intermediate of the valine synthesis pathway. However, neither natural microorganisms nor valine producing engineered strains have been reported yet to produce this chemical. Based on the 2,3-butanediol synthesis pathway, a biological route of 2,3-dihydroxyisovalerate production was developed using a budA and ilvD disrupted Klebsiella pneumoniae strain in our previous research. We hypothesised, that other 2,3-butanediol producing bacteria could be used for 2,3-dihydroxyisovalerate production. Here a budA disrupted Enterobacter cloacae was constructed, and this strain exhibited a high 2,3-dihydroxyisovalerate producing ability. Disruption of ilvD in E. cloacae ΔbudA further increased 2,3-dihydroxyisovalerate level. The disruption of budA, encoding an acetolactate decarboxylase, resulted in the acetolactate synthesized in the 2,3-butanediol synthesis pathway to flow into the valine synthesis pathway. The additional disruption of ilvD, encoding a dihydroxy acid dehydratase, prevented the 2,3-dihydroxyisovalerate to be further metabolized in the valine synthesis pathway. Thus, the disruption of both budA and ilvD in 2,3-butanediol producing strains might be an universal strategy for 2,3-dihydroxyisovalerate accumulation. After optimization of the medium components and culture parameters 31.2 g/L of 2,3-dihydroxyisovalerate was obtained with a productivity of 0.41 g/L h and a substrate conversion ratio of 0.56 mol/mol glucose in a fed-batch fermentation. This approach provides an economic way for 2,3-dihydroxyisovalerate production.
| Type: | Article |
|---|---|
| Title: | Production of 2,3-dihydroxyisovalerate by Enterobacter cloacae |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.enzmictec.2020.109650 |
| Publisher version: | https://doi.org/10.1016/j.enzmictec.2020.109650 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Enterobacter cloacae; 2,3-dihydroxyisovalerate; 2,3-butanediol; branched-chain amino acid |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10109793 |
Archive Staff Only
 |
View Item |
