Lu, X;
Yao, Y;
Yang, Y;
Zhang, Z;
Gu, J;
Mojovic, L;
Knezevic-Jugovic, Z;
... Hao, J; + view all
(2020)
Ethylene glycol and glycolic acid production by wild-type Escherichia coli.
Biotechnology and Applied Biochemistry
, 68
(4)
pp. 744-755.
10.1002/bab.1987.
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Abstract
Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild‐type Escherichia coli W3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore, E. coli strains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced by E. coli ΔyqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, the E. coli ΔaldA+yqhD produced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production by E. coli on xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.
| Type: | Article |
|---|---|
| Title: | Ethylene glycol and glycolic acid production by wild-type Escherichia coli |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/bab.1987 |
| Publisher version: | https://doi.org/10.1002/bab.1987 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | ethylene glycol; glycolic acid; xylonic acid; xylose; E. coli |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10109792 |
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