Pereno, V;
Carugo, D;
Bau, L;
Sezgin, E;
de la Serna, JB;
Eggeling, C;
Stride, E;
(2017)
Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes.
ACS Omega
, 2
(3)
pp. 994-1002.
10.1021/acsomega.6b00395.
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Abstract
Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles. Currently, however, it is expensive and time-consuming compared with other methods. In this study, we demonstrate that 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes, such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis.
| Type: | Article |
|---|---|
| Title: | Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1021/acsomega.6b00395 |
| Publisher version: | https://doi.org/10.1021/acsomega.6b00395 |
| Language: | English |
| Additional information: | This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.http://creativecommons.org/licenses/by/4.0/ |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10109405 |
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