Spaull, R;
McPherson, B;
Gialeli, A;
Clayton, A;
Uney, J;
Heep, A;
Cordero-Llana, Ó;
(2019)
Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus.
International Journal of Developmental Neuroscience
, 73
(1)
pp. 59-65.
10.1016/j.ijdevneu.2019.01.004.
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Abstract
Background: Preterm infants are at risk of germinal matrix haemorrhage‐intraventricular haemorrhage (GMH‐IVH) which leads to post‐haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate‐severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long‐term outcome after GMH‐IVH. In recent years, endosome‐derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro‐RNAs. Methods: This study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro‐RNA analysis. Results: NTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal‐sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro‐RNAs miR‐9, miR‐17, miR‐26a, miR‐124 and miR‐1911 were detected within the exosome‐enriched fraction and profiled over time. Conclusion: This is the first reported characterisation of exosomes from the CSF of preterm infants with post‐haemorrhagic hydrocephalus.
| Type: | Article |
|---|---|
| Title: | Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ijdevneu.2019.01.004 |
| Publisher version: | https://doi.org/10.1016/j.ijdevneu.2019.01.004 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Exosome; Preterm infant; Cerebrospinal fluid; Intraventricular hemorrhage; Cerebral palsy; Micro RNA |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Neurosciences Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10109226 |
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