Investigation of supercritical fluid technology to produce dry particulate formulations of antibody fragments

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Investigation of supercritical fluid technology to produce dry particulate formulations of antibody fragments

Sarup, Louise Santha; (2004) Investigation of supercritical fluid technology to produce dry particulate formulations of antibody fragments. Doctoral thesis (Ph.D.), University College London (United Kingdom). Green open access

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Abstract

Micro, submicrometre and ultrafine particles are increasingly in demand as they have a broad application in pharmaceutical delivery and needle-free powder injection. Conventional methods of powder preparation including freeze-drying and spray drying have considerable disadvantages for these applications. The use of supercritical fluids for materials' processing is one of the efficient and novel approaches used to achieve high purity micron-sized particles in a single step. The technique of Solution Enhanced Dispersion by Supercritical Fluids (SEDS) dramatically reduces or eliminates the problems associated with current, conventional particle-formation and size-reduction processes. SEDS involves the rapid dispersion, mixing and extraction of a protein solution, anti-solvent and supercritical carbon dioxide. SEDS has been used to produce dry particulate formulations of three antibody fragments (D1.3Fv, 4D5Fab, PEG-4D5Fab) and a whole antibody (CDP850). The aim of this research was to assess affects of the SEDS process on the functionality antibody fragments and antibody. The results demonstrated that dried formulations of antibody fragments and antibody can be produced using the SEDS process. The level of damage caused during SEDS was found to be dependent on choice solvent and protein. The main cause of activity loss was identified as the presence of both water and solvent during SEDS processing.

Type:	Thesis (Doctoral)
Qualification:	Ph.D.
Title:	Investigation of supercritical fluid technology to produce dry particulate formulations of antibody fragments
Open access status:	An open access version is available from UCL Discovery
Language:	English
Additional information:	Thesis digitised by ProQuest.
Keywords:	(UMI)AAIU643906; Health and environmental sciences; Dry particulate formulations
URI:	https://discovery.ucl.ac.uk/id/eprint/10104887