Sindeeva, OA;
Kopach, O;
Kurochkin, MA;
Sapelkin, A;
Gould, DJ;
Rusakov, DA;
Sukhorukov, GB;
(2020)
Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells.
Frontiers in Bioengineering and Biotechnology
, 8
, Article 497. 10.3389/fbioe.2020.00497.
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Abstract
The patterned microchamber arrays based on biocompatible polymers are a versatile cargo delivery system for drug storage and site-/time-specific drug release on demand. However, functional evidence of their action on nerve cells, in particular their potential for enabling patterned neuronal morphogenesis, remains unclear. Recently, we have established that the polylactic acid (PLA)-based microchamber arrays are biocompatible with human cells of neuronal phenotype and provide safe loading for hydrophilic substances of low molecular weight, with successive site-specific cargo release on-demand to trigger local cell responses. Here, we load the nerve growth factor (NGF) inside microchambers and grow N2A cells on the surface of patterned microchamber arrays. We find that the neurite outgrowth in local N2A cells can be preferentially directed towards opened microchambers (upon-specific NGF release). These observations suggest the PLA-microchambers can be an efficient drug delivery system for the site- specific delivery of neuropeptides on-demand, potentially suitable for the migratory or axonal guidance of human nerve cells.
| Type: | Article |
|---|---|
| Title: | Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fbioe.2020.00497 |
| Publisher version: | https://doi.org/10.3389/fbioe.2020.00497 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Multidisciplinary Sciences, Science & Technology - Other Topics, polylactic acid (PLA), patterned microchamber array, drug delivery system, NGF, human N2A cells, NERVE GROWTH-FACTOR, GOLD, NANORODS, RELEASE, NANOSCALE, NANOPARTICLES, FEMTOSECOND, TOPOGRAPHY, OUTGROWTH |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10104815 |
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