Marshall, Alison Shona;
(2004)
The role of chemokines in oral lichen planus.
Doctoral thesis (Ph.D), University College London.
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Abstract
Oral lichen planus (OLP) is an inflammatory disease characterised by a localised infiltration of T cells in a band-like structure below the oral epithelium. Chemokines are secreted proteins known for their ability to attract leucocyte populations through specific receptors on these cells. In this study, five chemokines that are known to attract memory T cell populations were investigated in order to establish their possible role in the pathogenesis of OLP. mRNA and protein of the studied chemokines (monokine induced by interferon-gamma (MIG), interferon-induced protein-10 (IP-10), interferon-induced T cell attractant chemokine (I-TAC), cutaneous T cell attractant chemokine (CTACK) and macrophage inflammatory protein-3alpha (MIP- 3α)) was expressed by oral epithelial cells either constitutively or expressed following pro-inflammatory stimulation. IP-10 mRNA expression by oral epithelial cells was found to be enhanced by lipopolysaccharide stimulation, and IP-10 (and other relevant chemokines) were shown to have an anti-microbial effect against Streptococcus sanguis, perhaps suggesting that IP-10 is produced in OLP in response to, and/or to act against, an oral bacterial imbalance. It was confirmed that IP-10 and CTACK predominately attract memory T cells, however, CTACK produced from oral epithelial cells was more effective at chemoattraction after cytokine pre-treatment. mRNA transcripts of the CXC ELR- chemokines and CTACK were found to be upregulated in OLP lesional tissue in comparison to normal oral mucosal tissue. However, mRNA expression of MIP-3α or its receptor was not upregulated. The presence of CD40 and CD154 (whose ligation can enhance the production of some chemokines in epithelial cells) was demonstrated in oral lichen planus. In summary, chemokines are likely to play an important role in the migration of large numbers of T cells witnessed within OLP lesions. The exact initiating and precipitation factors accounting for the chronicity of OLP are not known but might include bacterial stimulation of chemokines.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | The role of chemokines in oral lichen planus. |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Thesis digitised by ProQuest |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10101875 |
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