Mohamad, Che Muhaya Haji; (1999) Lymphocytes and cytokines in ocular fluids in human intraocular inflammation. Doctoral thesis (Ph.D), UCL (University College London).

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Abstract

Many forms of uveitis have an unknown aetiology but are believed to be due to dysregulation of the immune system either locally in the eye or as part of a systemic disorder. Purpose: To ascertain the cellular phenotypes and cytokine pattern in the AH of patients with uveitis and pattern of cytokine production by T cells infiltrating the intraocular compartment. Methods: The AH and PB of patients with different types of uveitis (n=59) and healthy controls (n=10) were characterised according to the cellular phenotypes by three colour flow cytometry, and levels of T cell-derived and related cytokines quantitated by ELIS As. T cell lines were obtained by expansion of T cells in the VH and PB of FHC and lU patients and the ex vivo cytokine production by these cell lines determined. Results: There was a selective elevation of CD4+ T cells in the AH compared to PB in all types of uveitis with the exception of FHC, where there was a selective increase in CD8+ T cells. There was a significantly lower level of regulatory cytokine IL-10 in the AH of uveitis compared to control patients. T cell lines derived from the VH of patients with FHC and lU showed high production of type-1 cytokines (IL-2, IFNγ) and low production of type-2 cytokine, IL-4 following stimulation with a non-specific T cell mitogen, phytohaemaglutinin-A (PHA). The production of regulatory cytokine IL-10 was also low in these lines. Conclusions: The results further confirm that Th 1 cells are important in the pathogenesis of uveitis and that the presence of high level of IL-10 in the AH appears to have a protective effects against ocular morbidity. This knowledge is useful in planning future immunotherapeutic strategies to avoid use steroids and thus avoiding its well-known side effects.

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