Monteagudo, Juan Ignacio Mata;
(1997)
Fission yeast cell polarity.
Doctoral thesis (Ph.D.), University College London (United Kingdom).
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Abstract
Fission yeast cells are cylindrical. New cell wall materials are deposited only at the poles of the cell, in such a way that cells elongate while keeping both ends directly opposite each other. Mutants in the teal, teal and ban5 genes select growing sites which are not exactly at cell ends. As a result, the poles of the cell are no longer kept opposite, resulting in the production of bent cells and, in more extreme cases, in the formation of branches. The aim of the experiments described in this thesis was to understand the mechanisms by which fission yeast cells select growth sites at the ends of the cell. To do this, I have characterised cytologically and genetically mutants in the teal, teal and ban5 genes, and I have carried out a molecular analysis of the teal gene. The initial study of the mutants revealed abnormalities in the cytoskeleton and cell cycle dependencies in the appearance of the mutant phenotype. To carry out a molecular analysis of teal, the gene was cloned using positional cloning. Both deletion and overexpression of the gene cause a cell shape defect in which cells bend and branch. Antibodies raised against the teal protein were used to determine the intracellular localisation of teal, teal is located at the cell poles Microtubules are continuously required to transfer teal to the cell ends, and teal is located at the ends of microtubules growing towards the cell poles. I propose that teal acts as an end marker, directing the growth machinery to the cell poles. Teal is down-regulated in cells treated with pheromone, which grow towards a mating partner and no longer maintain their ends exactly opposed, teal may also influence microtubular organisation affecting the maintenance of a single central axis.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D. |
| Title: | Fission yeast cell polarity |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Thesis digitised by ProQuest. |
| Keywords: | (UMI)AAI10045790; Biological sciences; Fission; Yeast cell |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10098961 |
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