Bukreeva, I;
Asadchikov, V;
Buzmakov, A;
Chukalina, M;
Ingacheva, A;
Korolev, NA;
Bravin, A;
... Cedola, A; + view all
(2020)
High resolution 3D visualization of the spinal cord in a post-mortem murine model.
Biomedical Optics Express
, 11
(4)
pp. 2235-2253.
10.1364/BOE.386837.
Preview |
Text
boe-11-4-2235.pdf - Published Version Download (13MB) | Preview |
Abstract
A crucial issue in the development of therapies to treat pathologies of the central nervous system is represented by the availability of non-invasive methods to study the three-dimensional morphology of spinal cord, with a resolution able to characterize its complex vascular and neuronal organization. X-ray phase contrast micro-tomography enables a high-quality, 3D visualization of both the vascular and neuronal network simultaneously without the need of contrast agents, destructive sample preparations or sectioning. Until now, high resolution investigations of the post-mortem spinal cord in murine models have mostly been performed in spinal cords removed from the spinal canal. We present here post-mortem phase contrast micro-tomography images reconstructed using advanced computational tools to obtain high-resolution and high-contrast 3D images of the fixed spinal cord without removing the bones and preserving the richness of micro-details available when measuring exposed spinal cords. We believe that it represents a significant step toward the in-vivo application.
| Type: | Article |
|---|---|
| Title: | High resolution 3D visualization of the spinal cord in a post-mortem murine model |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1364/BOE.386837 |
| Publisher version: | https://doi.org/10.1364/BOE.386837 |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10098319 |
Archive Staff Only
 |
View Item |
