Bafna, JA;
Sans-Serramitjana, E;
Acosta-Gutierrez, S;
Bodrenko, IV;
H Ouml R Ouml Mp Ouml Li, D;
Berscheid, A;
Broetz-Oesterhelt, H;
... Ceccarelli, M; + view all
(2020)
Kanamycin uptake into Escherichia coli is facilitated by OmpF and OmpC porin channels located in the outer membrane.
ACS Infectious Diseases
, 6
(7)
pp. 1855-1865.
10.1021/acsinfecdis.0c00102.
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Abstract
Despite decades of therapeutic application of aminoglycosides, it is still a matter of debate if porins contribute to the translocation of the antibiotics across the bacterial outer membrane. Here, we quantified the uptake of kanamycin across the major porin channels OmpF and OmpC present in the outer membrane of Escherichia coli. Our analysis revealed that, despite its relatively large size, about 10–20 kanamycin molecules per second permeate through OmpF and OmpC under a 10 μM concentration gradient, whereas OmpN does not allow the passage. Molecular simulations elucidate the uptake mechanism of kanamycin through these porins. Whole-cell studies with a defined set of E. coli porin mutants provide evidence that translocation of kanamycin via porins is relevant for antibiotic potency. The values are discussed with respect to other antibiotics.
| Type: | Article |
|---|---|
| Title: | Kanamycin uptake into Escherichia coli is facilitated by OmpF and OmpC porin channels located in the outer membrane |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1021/acsinfecdis.0c00102 |
| Publisher version: | https://doi.org/10.1021/acsinfecdis.0c00102 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | E. coli OmpF OmpC OmpN bacterial porins optimal transport |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10097708 |
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