Nichols, JM;
Antolovic, V;
Reich, JD;
Brameyer, S;
Paschke, P;
Chubb, JR;
(2020)
Cell and molecular transitions during efficient dedifferentiation.
eLife
, 9
10.7554/eLife.55435.
(In press).
Preview |
Text
elife-55435-v1.pdf - Accepted Version Download (15MB) | Preview |
Abstract
Dedifferentiation is a critical response to tissue damage, yet is not well understood, even at a basic phenomenological level. Developing Dictyostelium cells undergo highly efficient dedifferentiation, completed by most cells within 24 hours. We use this rapid response to investigate the control features of dedifferentiation, combining single cell imaging with high temporal resolution transcriptomics. Gene expression during dedifferentiation was predominantly a simple reversal of developmental changes, with expression changes not following this pattern primarily associated with ribosome biogenesis. Mutation of genes induced early in dedifferentiation did not strongly perturb the reversal of development. This apparent robustness may arise from adaptability of cells: the relative temporal ordering of cell and molecular events was not absolute, suggesting cell programmes reach the same end using different mechanisms. In addition, although cells start from different fates, they rapidly converged on a single expression trajectory. These regulatory features may contribute to dedifferentiation responses during regeneration.
| Type: | Article |
|---|---|
| Title: | Cell and molecular transitions during efficient dedifferentiation |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.7554/eLife.55435 |
| Publisher version: | https://doi.org/10.7554/eLife.55435 |
| Language: | English |
| Additional information: | © 2020, Nichols et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | developmental biology, dictyostelium, regenerative medicine, stem cells |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10095460 |
Loading...
Loading...Loading...Loading...Archive Staff Only
 |
View Item |
