King, CM;
Bohmbach, K;
Minge, D;
Delekate, A;
Zheng, K;
Reynolds, J;
Rakers, C;
... Henneberger, C; + view all
(2020)
Local Resting Ca2+ Controls the Scale of Astroglial Ca2+ Signals.
Cell Reports
, 30
(10)
3466-3477.e4.
10.1016/j.celrep.2020.02.043.
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Abstract
Astroglia regulate neurovascular coupling while engaging in signal exchange with neurons. The underlying cellular machinery is thought to rely on astrocytic Ca2+ signals, but what controls their amplitude and waveform is poorly understood. Here, we employ time-resolved two-photon excitation fluorescence imaging in acute hippocampal slices and in cortex in vivo to find that resting [Ca2+] predicts the scale (amplitude) and the maximum (peak) of astroglial Ca2+ elevations. We bidirectionally manipulate resting [Ca2+] by uncaging intracellular Ca2+ or Ca2+ buffers and use ratiometric imaging of a genetically encoded Ca2+ indicator to establish that alterations in resting [Ca2+] change co-directionally the peak level and anti-directionally the amplitude of local Ca2+ transients. This relationship holds for spontaneous and for induced (for instance by locomotion) Ca2+ signals. Our findings uncover a basic generic rule of Ca2+ signal formation in astrocytes, thus also associating the resting Ca2+ level with the physiological “excitability” state of astroglia.
| Type: | Article |
|---|---|
| Title: | Local Resting Ca2+ Controls the Scale of Astroglial Ca2+ Signals |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.celrep.2020.02.043 |
| Publisher version: | https://doi.org/10.1016/j.celrep.2020.02.043 |
| Language: | English |
| Additional information: | © 2020 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) |
| Keywords: | astrocytes, brain, calcium signalling, calcium stores, cortex, fluorescence microscopy, hippocampus, in vivo, locomotion, mechanism, quantitative |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10093561 |
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