Desfougères, Y;
Saiardi, A;
(2020)
Dictyostelium discoideum as a Model to Study Inositol Polyphosphates and Inorganic Polyphosphate.
Methods in Molecular Biology
, 2091
pp. 59-71.
10.1007/978-1-0716-0167-9_5.
Preview |
Text
MiMB RPS.pdf - Accepted Version Download (1MB) | Preview |
Abstract
The yeast Saccharomyces cerevisiae has given us much information on the metabolism and function of inositol polyphosphates and inorganic polyphosphate. To expand our knowledge of the metabolic as well as functional connections between inositol polyphosphates and inorganic polyphosphate, we have refined and developed techniques to extract and analyze these molecules in a second eukaryotic experimental model, the amoeba Dictyostelium discoideum. This amoeba, possessing a well-defined developmental program, is ideal to study physiological changes in the levels of inositol polyphosphates and inorganic polyphosphate, since levels of both molecules increase at late stages of development. We detail here the methods used to extract inositol polyphosphates using perchloric acid and inorganic polyphosphate using acidic phenol. We also present the postextraction procedures to visualize and quantify these molecules by polyacrylamide gel electrophoresis and by malachite green assay.
| Type: | Article |
|---|---|
| Title: | Dictyostelium discoideum as a Model to Study Inositol Polyphosphates and Inorganic Polyphosphate |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-0167-9_5 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-0167-9_5 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | DAPI staining, Electrophoresis, Extraction, Inositol polyphosphate, Inositol pyrophosphate, Phytic acid, Toluidine blue, polyP |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10091924 |
Archive Staff Only
 |
View Item |
